Enzymatic DNA Synthesis
Choose the DNA format that best fits your project. Cell-free enzymatic DNA synthesis is the latest addition to our service portfolio. Supporting you with greater flexibility in DNA formats for gene therapy, gene editing and further advanced therapy projects.
Cell-free Enzymatic DNA Synthesis
Expanding the range of DNA solutions for advanced therapies
Biomay has expanded its DNA manufacturing portfolio with the introduction of cell-free, enzymatically synthesized DNA. This DNA technology is fully integrated into the comprehensive end-to-end GMP service offering. It is meant to support our customer’s gene therapy, gene editing and further projects. At the same time, it provides more flexibility by complementing the benefits of Biomay’s plasmid DNA solutions.
Comprehensive offering of relevant DNA manufacture technologies
Keeping pace with evolving market needs requires continuous investment in technology and manufacturing capabilities. Biomay is committed to advancing its technology portfolio and developing new solutions that expand customer choice and address emerging requirements in the development and manufacturing of advanced therapeutics.
Enzymatically synthesized DNA offers a complementary approach to established plasmid DNA technologies and has the potential for applications across a wide range of advanced therapy and nucleic acid platforms. These include direct non-viral DNA vaccines or gene therapeutics, viral vector production for adeno-associated virus (AAV) and lentiviral vector (LV) applications, as well as DNA templates for mRNA production (in vitro transcription, IVT) or gene editing (homology-directed repair, HDR).
As a cell-free manufacturing approach, the technology eliminates the need for bacterial fermentation and avoids bacterial plasmid backbones. This may offer advantages in process design, impurity management and manufacturing lead times, with potential benefits for cost of goods (COGs) and personalized manufacturing applications.
Our cell-free, enzymatic DNA synthesis platform:
Our proprietary platform enables the production of three distinct DNA formats. This versatility supports a broad range of applications.
| Format | Structure | Application |
|---|---|---|
| Linear closed DNA |  | DNA Vaccine Viral vector production (AAV, LV) |
| Linear one-end-closed DNA |  | DNA template for mRNA (IVT) |
| Linear open-ended DNA |  | Gene editing (homology directed repair, HDR) |
The cell-free enzymatic in-vitro process combines rolling circle amplification (RCA) with proprietary processing of the hairpin loops to generate the desired DNA modality, followed by downstream purification, aseptic filling and quality control.
Services offered for cell-free enzymatic DNA synthesis (linear synthetic DNA):
- Linear synthetic DNA design and construction
- Process development / feasibility studies
- Analytical development / QC method qualification
- HQ or GMP manufacturing of linear synthetic DNA
- QC testing and stability studies
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