Cas9 mRNA

(RUO or GMP)

Webshop

Biomay Cas9-mRNA Shop

Biomay’s premade CRISPR/Cas9 mRNA variants are available as off-the-shelf products in both Research Use Only (RUO) and GMP-compliant grades. This supports the seamless transition from R&D to clinical development.

Derived from wild-type Cas9 nuclease of Streptococcus pyogenes, Biomay’s Cas9 mRNA portfolio includes multiple optimized formats—featuring distinct 5′ cap analogs, poly(A) tail lengths, and nucleoside modifications. These variants are engineered to meet the specific needs of genome editing applications, including T-cell receptor modification (TCR-T) and chimeric antigen receptor (CAR-T) cell engineering.

Biomay’s Cas9 mRNA is produced using a fully integrated, in-house GMP manufacturing platform supported by robust quality control and a panel of qualified analytical assays. Functional performance has been confirmed in cell-based assays, demonstrating equivalence to leading benchmark Cas9 mRNA products. A capping efficiency exceeding 95% supports enhanced mRNA stability, efficient translation, and sustained functional activity. Low double-stranded RNA (dsRNA) content and high product homogeneity contribute to reduced innate immune activation and consistent in vivo performance.

As an FDA-inspected manufacturer, Biomay is committed to delivering the highest quality RNA products, empowering clients from early research through to clinical-stage programs.

Figure 1: Cellular assay for Cas9 editing efficiency: Editing effciency was determinded in HEK293 cells using two different gRNAs for knockout of the AAVS1 locus, followed by Sanger sequencing. Editing efficieny of Biomay’s Cas9 mRNA is comparable to that of a reference Cas9 mRNA.

Features of Biomay's Cas9 mRNA

  • Available off-the-shelf
  • Quality grades: RUO (research use only) and GMP (clinical)
  • Seamless transition from pre-clinical to clinical
  • Proven activity
  • Highly pure
  • Various aliquots: 25 / 100 / 1000 µg (RUO)
  • Free RUO samples available when ordering GMP material
  • Shipping frozen on dry ice
  • Follow-up made-to-order possible

RUO-Grade Cas9 mRNA

 You can place your purchase order directly via our webshop below. We will contact you for requests, shipping and payment.
Shipping costs (€ 300 to destinations inside Europe, € 1,200 to destinations outside Europe) will be added to your invoice.

  • Cas9 mRNA (Cap4) RUO

    160-0025-RUO | 160-0100-RUO | 160-1000-RUO

    Wild type spCas9 mRNA with nuclear localization sequence (NLS-spCas9-NLS)

    € 220 – € 2,200

GMP-Grade Cas9 mRNA

 Biomay’s GMP grade Cas9 mRNA enable the seamless, time saving and cost efficient transfer of a project from pre-clinical stage to clinical research.

  • Cas9 mRNA (Cap4) GMP

    560-xxxx-GMP

    Wild type spCas9 mRNA with nuclear localization sequence (NLS-spCas9-NLS)

Biomay imposes no restrictions on the use of the offered Cas9 mRNA products. Depending on the application, end users may need to secure appropriate third-party licenses related to CRISPR/Cas-mediated gene editing or the use of modified nucleosides from the relevant intellectual property holders.

Biomay also offers mRNA made to order, please see our mRNA services  or contact us.

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