AAV Starting Plasmids

Webshop

Screenshot

Biomay offers a variety of pre-made starting plasmids for the packaging of recombinant adeno-associated virus (rAAV) as gene transfer vectors.
For packaging rAAV vectors in HEK293 cell culture, a typical co-transfection set consists of three plasmids:

      1. an adenovirus helper plasmid (Av helper for packaging)
      2. a Rep/Cap plasmid (defining the AAV replication and capsid serotype)
      3. a transgene plasmid (containing the gene of interest)

Transgene plasmids with the individual genes of interest are usually custom made. The adenovirus (Av) helper plasmid and the Rep/Cap plasmids however are generic and universally applicable.

Biomay’s Av helper plasmid pBM-AV-Helper has been designed to focus on the essential Av elements. With a minimized size of only 11 kbp, an optimized transfection rates is ensured.

The tissue specificity and organ targeting of an rAAV vector is defined by its capsid serotype. Biomay offers a variety of plasmids coding for different Rep/Cap serotypes whic are clinically significant.

Overview on available Rep/Cap Plasmids:

AAV plasmid name (map)
Rep sero­type
Cap sero­type
RUO grade Aliqout size in mg
GMP grade Aliqout size in mg
2
1
1 / 5 / 10
1 / 5 / 10
2
2
1 / 5 / 10
1 / 5 / 10
2
5
1 / 5 / 10
1 / 5 / 10
6
1 / 5 / 10
1 / 5 / 10
2
8
1 / 5 / 10
1 / 5 / 10
2
9
1 / 5 / 10
1 / 5 / 10
2
10
1 / 5 / 10
1 / 5 / 10

Features of Biomay's AAV Starting Plasmids:

  • Available off-the-shelf
  • Quality grades: RUO (Research Use Only) and GMP (clinical)
  • Seamless and time-saving transition from pre-clinical to clinical research
  • License and royalty free
  • Various aliquots (1, 5, 10 mg)
  • Free RUO samples available
  • Shipping as frozen solution on dry ice

RUO-Grade AAV Plasmids

You can place your purchase order directly via our webshop below. We will contact you for requests, shipping and payment.
 Shipping costs (€ 300 to destinations inside Europe, € 1,200 to destinations outside Europe) will be added to your invoice.

  • pBM-AV-Helper RUO

    100-01-RUO / 100-05-RUO / 100-10-RUO

    Adenovirus helper plasmid for manufacturing recombinant adeno-associated virus (AAV) particles; For research use only

     200 1,650
  • pBM-AAV1 RUO

    101-01-RUO / 101-05-RUO / 101-10-RUO

    DNA plasmid encoding Rep2/Cap1 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 1; For research use only

     120 980
  • pBMY-AAV2 RUO

    102-01-RUO / 102-05-RUO / 102-10-RUO

    DNA plasmid encoding Rep2/Cap2 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 2. For research use only

     120 980
  • pBM-AAV5 RUO

    105-01-RUO / 105-05-RUO / 105-10-RUO

    DNA plasmid encoding Rep2/Cap5 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 5; For research use only

     120 980
  • pBMY-AAV6 RUO

    106-01-RUO / 106-05-RUO / 106-10-RUO

    DNA plasmid encoding Rep2/Cap6 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 6. For research use only

     120 980
  • pBMY-AAV8 RUO

    108-01-RUO / 108-05-RUO / 108-10-RUO

    DNA plasmid encoding Rep2/Cap8 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 8. For research use only

     120 980
  • pBM-AAV9 RUO

    109-01-RUO / 109-05-RUO / 109-10-RUO

    DNA plasmid encoding Rep2/Cap9 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 9. For research use only

     120 980
  • pBM-AAVrh10 RUO

    110-01-RUO / 110-05-RUO / 110-10-RUO

    DNA plasmid encoding Rep2/Caprh10 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype rh10; For research use only

     120 980

In case you have a special request:

GMP-Grade AAV Plasmids

Biomay’s GMP grade AAV plasmids enable the seamless, time saving and cost efficient transfer of an AAV project from non-clinical to clinical research.

Features of Biomay's GMP Grade AAV Catalog Plasmids:

  • Immediate availability of clinical grade material
  • Produced under GMP conditions (clinical drug substance grade)
  • Quality control with qualified analytical assays
  • Quality management following EMA law (directive 2001/83/EC)
  • Qualified Person released
  • Comes with CoA, CoC, TSE-statement
  • Comes with batch summary report
  • Facilitates follow-on custom manufacturing (late-stage, large-scale)
  • 0.2 µm filtered and filled in various aliquot sizes
  • GMP conform shipping (dry ice, temperature monitored)
  • Ideal for direct application in GMP manufacturing of rAAV virus particles
  • pBM-AV-Helper GMP

    500-01-GMP / 500-05-GMP / 500-10-GMP

    Adenovirus helper plasmid for manufacturing recombinant adeno-associated virus (AAV) particles; cGMP

  • pBM-AAV1 GMP

    501-01-GMP / 501-05-GMP / 501-10-GMP

    DNA plasmid encoding Rep2/Cap1 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 1; cGMP

  • pBMY-AAV2 GMP

    502-01-GMP / 502-05-GMP / 502-10-GMP

    DNA plasmid encoding Rep2/Cap2 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 2; cGMP

  • pBM-AAV5 GMP

    505-01-GMP / 505-05-GMP / 505-10-GMP

    DNA plasmid encoding Rep2/Cap5 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 5; cGMP

  • pBMY-AAV6 GMP

    506-01-GMP / 506-05-GMP / 506-10-GMP

    DNA plasmid encoding Rep2/Cap6 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 6; cGMP

  • pBMY-AAV8 GMP

    508-01-GMP / 508-05-GMP / 508-10-GMP

    DNA plasmid encoding Rep2/Cap8 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 8; cGMP

  • pBM-AAV9 GMP

    509-01-GMP / 509-05-GMP / 509-10-GMP

    DNA plasmid encoding Rep2/Cap9 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype 9; cGMP

  • pBM-AAVrh10 GMP

    510-01-GMP / 510-05-GMP / 510-10-GMP

    DNA plasmid encoding Rep2/Caprh10 genes of adeno-associated virus (AAV), intended for preparation of AAV particles of serotype rh10; cGMP

In case you have a special request: