Lentivirus (LV) Starting Plasmids
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Biomay offers a variety of ready-to-use starting plasmids for packaging of recombinant lentiviral vectors (rLV) as gene transfer vectors.
Lentiviral vectors derived from the human immunodeficiency virus 1 (HIV-1) are well characterized and established gene delivery systems. Due to the large payload, they have become a widely used tool in cell and gene therapies for stable expression of therapeutic genes. In particular, chimeric antigen receptor (CAR) T-cell therapy is a common application of lentiviral vectors.
For packaging recombinant lentiviral vectors in HEK293 cell culture, a set of the following plasmids is required:
- Envelope plasmid providing the envelope glycoprotein, which is typically derived from the vesicular stomatitis virus glycoprotein G (VSV-G) providing a broad tropism and allowing the virus to infect a wide range of cell types
- Packaging plasmids containing genes coding for structural proteins (gag), functional enzymes (pol) and expression regulators (rev)
- Transgene plasmid containing the gene of interest flanked by long terminal repeat (LTR) sequences, which facilitate the integration of the transgene sequence into the host genome
Transgene plasmids with the individual genes of interest are usually custom made. The lentiviral envelope plasmid and the packaging plasmids, however, are generic and universally applicable.
Biomay provides a full set of lentiviral plasmids (envelope, packaging and reporter plasmids). The plasmids are compatible with the most widely used systems currently on the market, including 2nd (three-plasmid-system) and 3rd generation packaging system (four-plasmid-system).
The 2nd generation lentiviral vector system consists of 3 plasmids which makes it easier to handle and to transfect than the 3rd generation system. Therefore, it often gives higher viral yields due to less complexity. The 3rd generation lentiviral vector system consists of 4 plasmids, giving greater control and customization over viral components. Due to the increased biosafety, it is preferred for clinical and therapeutic applications. The lentiviral envelope plasmid (pBM-LV-VSVG) is generic and can be used in both generations.
Biomay’s 3rd generation lentiviral plasmids
| Plasmid (link to map) | Description | RUO grade aliquot size in mg | GMP grade aliqout size in mg | Link to data sheet |
|---|---|---|---|---|
| pBM-LV-VSVG | LV envelope plasmid encoding VSV-G | 1 / 5 / 10 | on request | Data Sheet |
| pBM-LV-GagPol | LV packaging plasmid encoding gag and pol | 1 / 5 / 10 | on request | Data Sheet |
| pBM-LV-Rev | LV packaging plasmid encoding rev | 1 / 5 / 10 | on request | Data Sheet |
| pBM-LV-eGFP | LV reporter transgene plasmid encoding eGFP | 1 / 5 / 10 | N/A | Data Sheet |
Biomay’s 2nd generation lentiviral plasmids
| Plasmid (link to map) | Description | RUO grade aliquot size in mg | GMP grade aliqout size in mg | Link to data sheet |
|---|---|---|---|---|
| pBM-LV-VSVG | LV envelope plasmid encoding VSV-G | 1 / 5 / 10 | on request | Data Sheet |
| pBM-LV-Pac | LV packaging plasmid encoding gag, pol, tat and rev | 1 / 5 / 10 | on request | Data Sheet |
| pBM-LV-eGFP | LV reporter transgene plasmid encoding eGFP | 1 / 5 / 10 | N/A | Data Sheet |
Features of Biomay's Starting Plasmids:
- Available off-the-shelf
- Quality grades: RUO (Research Use Only) and GMP (clinical)
- Seamless and time-saving transition from pre-clinical to clinical research
- License and royalty free
- Various aliquots (1, 5, 10 mg)
- Free RUO samples available when ordering GMP samples
- Shipping as frozen solution on dry ice
RUO-Grade Lentivirus Plasmids
You can place your purchase order directly via our webshop below. We will contact you for requests, shipping and payment. Shipping charges for EU destinations start at €300, and for non-EU destinations start at €1,200. Final shipping costs are calculated based on the delivery location and will be confirmed prior to order fulfilment.
In case you have a special request:
See our > Terms and Conditions.
GMP-Grade Lentivirus Plasmids
Biomay’s GMP grade AAV plasmids enable the seamless, time saving and cost efficient transfer of an AAV project from non-clinical to clinical research.
Features of Biomay's GMP Grade AAV Catalog Plasmids:
- Immediate availability of clinical grade material
- Produced under GMP conditions (clinical drug substance grade)
- Quality control with qualified analytical assays
- Quality management following EMA law (directive 2001/83/EC)
- Qualified Person released
- Comes with CoA, CoC, TSE-statement
- Comes with batch summary report
- Facilitates follow-on custom manufacturing (late-stage, large-scale)
- 0.2 µm filtered and filled in various aliquot sizes
- GMP conform shipping (dry ice, temperature monitored)
- Ideal for direct application in GMP manufacturing of rAAV virus particles
In case you have a special request: